Biochemistry

Effect of heavy-metal ions on the activity of enzymes

Glycolysis (temperature measurement)

Principle

Investigating the temperature increase during the fermentation of sugar by yeast cells.

The enzymatic activity of catalase

Principle

To examine the enzymatic decompositionof hydrogen peroxide, a cell respiratorypoison, in the liver.

Glycolysis (pressure measurement)

Principle

The aim of this experiment is to prove glycolysis by means of measuring the CO2 production under various
experimental conditions (temperature, pH).

Determination of the Michaelis constant

Principle

The enzymatic hydrolysis of urea in an aquous solution yields carbon dioxide and ammonia. The ions of
these compounds increase the conductivity of the solution. The speed of urea hydrolysis, which is
caused by the urease enzyme, can be de ...

Substrate inhibition of enzymes

Principle

The enzymatic hydrolysis of urea in aqueous solution liberates carbondioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements can therefore be used to measure the rate of hydrolysis of urea ...

Enzyme inhibition (poisoning of enzymes)

Principle

The enzymatic hydrolysis of urea in aqueous solution liberates carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements enable the rate of hydrolysis of urea by the enzyme urease to b ...

Determination of the isoelectric point of an amino acid (glycine) (with the Cobra3 Basic-Unit)

Principle

Amino acid molecules carry both acid and amino groups. They can therefore form both acidic anions and basic cations. The pH at which these two types of iones are both present in the same concentration is called the isoelectric point.

Tasks

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Determination of the Michaelis constant (with the Cobra3 Basic-Unit)

Principle

The enzymatic hydrolysis of urea in aqueous solution liberates carbon dixide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements can so be made to determine the rate of hydrolysis of urea by the enzyme urease ...