Determination of the isoelectric point of an amino acid (glycine) (with the Cobra3 Basic-Unit)

Product No: P4120111

Principle

Amino acid molecules carry both acid and amino groups. They can therefore form both acidic anions and basic cations. The pH at which these two types of iones are both present in the same concentration is called the isoelectric point.

Tasks

  1. This isoelectric point is to be determined by recording the titration curve for the amino acid glycine.

What you can learn about

  • Isoelectric point
  • Acidic anions
  • Basic cations
  • Zwitterions
  • Equivalence (inflection) points
  • pKs value
  • Titration
  • Motor piston burette



Materiallist (Excerpt) Product Amount
Motor.piston burette,univer.50ml 36499-93 1
Cobra3 BASIC-UNIT, USB 12150-50 1
Measuring module pH/potential 12101-00 1
Magnetic stirrer Mini / MST 47334-93 1
pH-electrode, polythene sheathed 18450-00 1
Cobra3, sensor -20..110 C 12120-00 1
Power supply 12V / 2A 12151-99 1
Cable Basic-Unit/Motor piston burette 36501-03 1
Software Cobra3 pH and potential 14509-61 1
Caustic soda sol.,1.0M 1000 ml 48329-70 1
Hydrochloric acid, 1.0 mol/l, 1000 ml 48454-70 1
Water, distilled 5 l 31246-81 1
Buffer solution, pH 4.01, 1000 ml 46270-12 1
Buffer solution, pH 10.01,1000 ml 46272-12 1
Glycocoll /glycine/ 100 g 31341-10 1
Volumetric flask 500 ml, IGJ19/26 36551-00 1
Pipettor 36592-00 1
Volumetric pipette, 50 ml 36581-00 1
Glass beaker DURAN®, tall, 250 ml 36004-00 1
Glass beaker DURAN®, tall, 50 ml 36001-00 3
Spoon, special steel 33398-00 1
Funnel, glass, top dia. 55 mm 34457-00 1
Magnetic stirring bar 30 mm, cylindrical 46299-02 1
Wash bottle, plastic, 500 ml 33931-00 1
Rubber stopper,d=18/14mm, 1 hole 39254-01 1

Literature for this article als follows

Title Product Language
TESS expert Handbook Laboratory Experiments Biology 16506-02 ENG

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Product No: P4120111

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