The enzymatic hydrolysis of urea in aqueous solution liberates
carbon dixide and ammonia. The ions of these compounds increase the
conductivity of the solution. Conductivity measurements can so be
made to determine the rate of hydrolysis of urea by the enzyme
urease at various substrate concentrations.
| Materiallist (Excerpt) |
Product |
Amount |
|
Cobra3 BASIC-UNIT, USB
|
12150-50
|
1
|
|
Measuring module Conductivity
|
12108-00
|
1
|
|
Conductivity probe K1
|
18151-02
|
1
|
|
Urease soln.in 50% glycerol,10ml
|
31924-03
|
1
|
|
Magnetic stirrer Mini / MST
|
47334-93
|
1
|
|
Cobra3, sensor -20..110 C
|
12120-00
|
1
|
|
Micro-l syringe, 100 micro-l
|
02606-00
|
1
|
|
Power supply 12V / 2A
|
12151-99
|
1
|
|
Support base variable
|
02001-00
|
1
|
|
Software Cobra3 Conductivity
|
14508-61
|
1
|
|
Water, distilled 5 l
|
31246-81
|
1
|
|
Urea, 250 g
|
30086-25
|
1
|
|
Erlenmeyer flask, narrow neck, 100 ml, PN 19
|
36418-00
|
6
|
|
Separator f.magnetic bars
|
35680-03
|
1
|
|
Pipettor
|
36592-00
|
1
|
|
Universal clamp
|
37718-00
|
1
|
|
Boss head
|
02043-00
|
2
|
|
Pasteur pipettes, 250 pcs
|
36590-00
|
1
|
|
Support rod, stainless steel, 500 mm
|
02032-00
|
1
|
|
Rubber caps, 10 pcs
|
39275-03
|
1
|
|
Glass beaker DURAN®, tall, 100 ml
|
36002-00
|
6
|
|
Volumetric pipette, 20 ml
|
36579-00
|
1
|
|
Microspoon, steel
|
33393-00
|
1
|
|
Magnetic stirring bar 30 mm, cylindrical
|
46299-02
|
1
|
|
Wash bottle, plastic, 500 ml
|
33931-00
|
1
|
|
Rubber stopper, d=22/17 mm, without hole
|
39255-00
|
6
|
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