The enzymatic hydrolysis of urea in aqueous solution liberates carbon dixide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements can so be made to determine the rate of hydrolysis of urea by the enzyme urease at various substrate concentrations.
| Materiallist (Excerpt) |
Product |
Amount |
|
Cobra3 Chem-Unit, USB
|
12153-50
|
1
|
|
Conductivity temperature probe Pt1000
|
13701-01
|
1
|
|
Urease soln.in 50% glycerol,10ml
|
31924-03
|
1
|
|
Software Cobra3 Chem-Unit
|
14520-61
|
1
|
|
Magnetic stirrer Mini / MST
|
47334-93
|
1
|
|
Micro-l syringe, 100 micro-l
|
02606-00
|
1
|
|
Power supply 12V / 2A
|
12151-99
|
1
|
|
Support base variable
|
02001-00
|
1
|
|
Water, distilled 5 l
|
31246-81
|
1
|
|
Urea, 250 g
|
30086-25
|
1
|
|
Erlenmeyer flask, narrow neck, 100 ml, PN 19
|
36418-00
|
6
|
|
Data cable, plug/ socket, 9 pole
|
14602-00
|
1
|
|
Separator f.magnetic bars
|
35680-03
|
1
|
|
Pipettor
|
36592-00
|
1
|
|
Universal clamp
|
37718-00
|
1
|
|
Boss head
|
02043-00
|
2
|
|
Pasteur pipettes, 250 pcs
|
36590-00
|
1
|
|
Support rod, stainless steel, 500 mm
|
02032-00
|
1
|
|
Rubber caps, 10 pcs
|
39275-03
|
1
|
|
Glass beaker DURAN®, tall, 100 ml
|
36002-00
|
6
|
|
Volumetric pipette, 20 ml
|
36579-00
|
1
|
|
Microspoon, steel
|
33393-00
|
1
|
|
Magnetic stirring bar 30 mm, cylindrical
|
46299-02
|
1
|
|
Wash bottle, plastic, 500 ml
|
33931-00
|
1
|
|
Rubber stopper, d=22/17 mm, without hole
|
39255-00
|
6
|
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