The enzymatic hydrolysis of urea in aqueous solution liberates
carbon dioxide and ammonia. The ions of these compounds increase
the conductivity of the solution. Conductivity measurements enable
the rate of hydrolysis of urea by the enzyme urease to be
determined. The addition of an appropriate inhibitor poisons the
enzyme, so that it no longer converts substrate.
| Materiallist (Excerpt) |
Product |
Amount |
|
Software Cobra4 - multi-user licence
|
14550-61
|
1
|
|
Cobra4 Wireless-Link
|
12601-00
|
1
|
|
Cobra4 Sensor-Unit Conductivity+, Conductivity/ Temperature (Pt1000)
|
12632-00
|
1
|
|
Conductivity temperature probe Pt1000
|
13701-01
|
1
|
|
Urease soln.in 50% glycerol,10ml
|
31924-03
|
1
|
|
Cobra4 Wireless Manager
|
12600-00
|
1
|
|
Magnetic stirrer Mini / MST
|
47334-93
|
1
|
|
Micro-l syringe, 100 micro-l
|
02606-00
|
1
|
|
Silver nitrate, cryst. 15 g
|
30222-00
|
1
|
|
Retort stand, h 750 mm
|
37694-00
|
1
|
|
Water, distilled 5 l
|
31246-81
|
1
|
|
Urea, 250 g
|
30086-25
|
1
|
|
Separator f.magnetic bars
|
35680-03
|
1
|
|
Pipettor
|
36592-00
|
1
|
|
Universal clamp
|
37715-00
|
1
|
|
Boss head
|
02043-00
|
1
|
|
Glass beaker DURAN®, tall, 100 ml
|
36002-00
|
8
|
|
Glass beaker DURAN®, short , 250 ml
|
36013-00
|
1
|
|
Volumetric pipette, 20 ml
|
36579-00
|
1
|
|
Microspoon, steel
|
33393-00
|
1
|
|
Magnetic stirring bar 30 mm, cylindrical
|
46299-02
|
1
|
|
Wash bottle, plastic, 500 ml
|
33931-00
|
1
|
|
Volumetric pipette, 1 ml
|
36575-00
|
1
|
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