The enzymatic hydrolysis of urea in an aquous solution yields
carbon dioxide and ammonia. The ions of
these compounds increase the conductivity of the solution. The
speed of urea hydrolysis, which is
caused by the urease enzyme, can be determined via conductivity
measurements at different substrate
concentration levels. The resulting values can then be used for the
calculation of the Michaelis constant.
| Materiallist (Excerpt) |
Product |
Amount |
|
Software Cobra4 - multi-user licence
|
14550-61
|
1
|
|
Cobra4 Wireless-Link
|
12601-00
|
1
|
|
Cobra4 Sensor-Unit Conductivity+, Conductivity/ Temperature (Pt1000)
|
12632-00
|
1
|
|
Conductivity temperature probe Pt1000
|
13701-01
|
1
|
|
Urease soln.in 50% glycerol,10ml
|
31924-03
|
1
|
|
Cobra4 Wireless Manager
|
12600-00
|
1
|
|
Magnetic stirrer Mini / MST
|
47334-93
|
1
|
|
Micro-l syringe, 100 micro-l
|
02606-00
|
1
|
|
Retort stand, h 750 mm
|
37694-00
|
1
|
|
Water, distilled 5 l
|
31246-81
|
1
|
|
Urea, 250 g
|
30086-25
|
1
|
|
Erlenmeyer flask, narrow neck, 100 ml, PN 19
|
36418-00
|
6
|
|
Separator f.magnetic bars
|
35680-03
|
1
|
|
Pipettor
|
36592-00
|
1
|
|
Universal clamp
|
37715-00
|
1
|
|
Boss head
|
02043-00
|
1
|
|
Volumetric pipette, 50 ml
|
36581-00
|
1
|
|
Glass beaker DURAN®, tall, 100 ml
|
36002-00
|
6
|
|
Volumetric pipette, 20 ml
|
36579-00
|
1
|
|
Microspoon, steel
|
33393-00
|
1
|
|
Magnetic stirring bar 30 mm, cylindrical
|
46299-02
|
1
|
|
Wash bottle, plastic, 500 ml
|
33931-00
|
1
|
|
Beaker, 250 ml, low form, plastic
|
36013-01
|
1
|
|
Rubber stopper, d=22/17 mm, without hole
|
39255-00
|
6
|
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