The digestion of proteins begins in the stomach. The gastric juices contain the enzyme pepsin along with diluted hydrochloric acid, which activates the enzyme from its precursor, pepsinogen. In the experiment, boiled fish is added to test tubes with water, enzyme solution, hydrochloric acid plus a mixture of enzyme solution and hydrochloric acid. In order to simulate the body temperature, the test tubes are placed in a large glass beaker which serves as a simple water bath. After a few hours, it is clearly evident that proteins break down only in the preparation with both pepsin and hydrochloric acid.
| Materiallist (Excerpt) |
Product |
Amount |
|
Portable Balance, OHAUS JE120
|
48895-00
|
1
|
|
Butane burner, Labogaz 206 type
|
32178-00
|
1
|
|
Support base variable
|
02001-00
|
1
|
|
Pepsin powder,soluble 100 g
|
30181-10
|
1
|
|
Water, distilled 5 l
|
31246-81
|
1
|
|
Hydrochloric acid,approx.5% 250ml
|
30315-25
|
1
|
|
Support ring, i.d. 130mm,w.boss
|
37722-03
|
1
|
|
Pipettor,bulb,3 valves, 10ml max.
|
47127-01
|
1
|
|
Test tube rack f. 6 tubes, wood
|
37685-10
|
1
|
|
Support rod, stainless steel, 500 mm
|
02032-00
|
1
|
|
Students thermometer,-10...+110°C, l = 180 mm
|
38005-02
|
1
|
|
Glass beaker DURAN®, tall, 600 ml
|
36006-00
|
1
|
|
Graduated cylinder 100 ml
|
36629-00
|
1
|
|
Erlenmeyer flask,narrow n.,100 ml
|
36118-00
|
1
|
|
Test tube 160x16 mm, 10 pcs
|
37656-03
|
1
|
|
Wire gauze 160 mm x 160 mm, ceramic cen.
|
33287-01
|
1
|
|
Spoon,w.spatula end,18 cm,plastic
|
38833-00
|
1
|
|
Butane cartridge C206, without valve
|
47535-00
|
1
|
|
Graduated pipette 10 ml
|
36600-00
|
1
|
|
Graduated pipette, 1 ml
|
36595-00
|
1
|
|
Knife, stainless
|
33476-00
|
1
|
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