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Determination of the Michaelis constant with the Basic-Unit

Product No: P1369830

The enzymatic hydrolysis of urea inaqueous solution liberates carbon dioxide and ammonia. The ions of these compounds increase the conductivity of thesolution. Conductivity measurements canso be made to determine the rate of hydrolysis of urea by the enzyme urease atvarious substrate concentrations. TheMichaelis constant can then be calculated from these values.

Materiallist (Excerpt)	Product	Amount
Cobra3 BASIC-UNIT, USB	12150-50	1
Measuring module Conductivity	12108-00	1
Conductivity probe K1	18151-02	1
Urease soln.in 50% glycerol,10ml	31924-03	1
Magnetic stirrer Mini / MST	47334-93	1
Cobra3, sensor -20..110 C	12120-00	1
Micro-l syringe, 100 micro-l	02606-00	1
Retort stand, h 750 mm	37694-00	1
Power supply 12V / 2A	12151-99	1
Software Cobra3 Conductivity	14508-61	1
Water, distilled 5 l	31246-81	1
Urea, 250 g	30086-25	1
Erlenmeyer flask, narrow neck, 100 ml, PN 19	36418-00	6
Separator f.magnetic bars	35680-03	1
Pipettor	36592-00	1
Universal clamp	37715-00	1
Pasteur pipettes, 250 pcs	36590-00	1
Right angle clamp	37697-00	1
Rubber caps, 10 pcs	39275-03	1
Glass beaker DURAN®, tall, 100 ml	36002-00	6
Volumetric pipette, 20 ml	36579-00	1
Microspoon, steel	33393-00	1
Magnetic stirring bar 30 mm, cylindrical	46299-02	1
Wash bottle, plastic, 500 ml	33931-00	1
Rubber stopper, d=22/17 mm, without hole	39255-00	6