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Enzyme inhibition (poisoning of enzymes) with the Basic-Unit

Product No: P1370030

The enzymatic hydrolysis of urea inaqueous solution liberates carbon dioxide and ammonia. The ions of these compounds increase the conductivity of thesolution. Conductivity measurementsenable the rate of hydrolysis of urea bythe enzyme urease to be determined. Theaddition of an appropriate inhibitorpoisons the enzyme, so that it no longerconverts substrate.

Materiallist (Excerpt)	Product	Amount
Cobra3 BASIC-UNIT, USB	12150-50	1
Measuring module Conductivity	12108-00	1
Conductivity probe K1	18151-02	1
Urease soln.in 50% glycerol,10ml	31924-03	1
Magnetic stirrer Mini / MST	47334-93	1
Cobra3, sensor -20..110 C	12120-00	1
Micro-l syringe, 100 micro-l	02606-00	1
Silver nitrate, cryst. 15 g	30222-00	1
Retort stand, h 750 mm	37694-00	1
Power supply 12V / 2A	12151-99	1
Software Cobra3 Conductivity	14508-61	1
Water, distilled 5 l	31246-81	1
Urea, 250 g	30086-25	1
Separator f.magnetic bars	35680-03	1
Pipettor	36592-00	1
Universal clamp	37715-00	1
Pasteur pipettes, 250 pcs	36590-00	1
Right angle clamp	37697-00	1
Rubber caps, 10 pcs	39275-03	1
Glass beaker DURAN®, tall, 100 ml	36002-00	8
Volumetric pipette, 20 ml	36579-00	1
Microspoon, steel	33393-00	1
Magnetic stirring bar 30 mm, cylindrical	46299-02	1
Wash bottle, plastic, 500 ml	33931-00	1
Volumetric pipette, 1 ml	36575-00	1
Beaker, 250 ml, low form, plastic	36013-01	1