Kinetics of the inversion of saccharose

 Product No: P3050301

Principle

The inversion reaction of saccharose, which is catalysed by protons, produces invert sugar, which is a mixture of glucose and fructose. The reaction is accompanied by a change in the optical rotation of the system. Glucose rotates the polarisation plane of linearly polarised light to the right, while inverted sugar rotates it to the left. A half-shade polarimeter is used for the measurement of the change in the angle of rotation of polarised ligh during the inversion reaction of saccharose over time.

Tasks

  1. Determine the specific rotation of saccharose and lactose by measuring the rotation angle of solutions of various concentrations.
  2. Determine the rate constant of the inversion of saccharose.

What you can learn about

  • Reaction rate
  • First order reaction
  • Polarimetry
  • Optical rotation



Materiallist (Excerpt) Product Amount
Set of Precision Balance Sartorius CPA 623S and measure software, 230 V 49224-88 1
Half-shade polarimeter, 230 V AC 35906-93 1
Immersion thermostat Alpha A, 230 V 08493-93 1
Magnetic stirrer MR Hei-Standard 35750-93 1
Bath for thermostat, Makrolon 08487-02 1
External circulation set for thermostat Alpha A 08493-02 1
Retort stand, 210 mm × 130 mm, h = 500 mm 37692-00 1
Hydrochloric acid,f.1M soln.amp. 30271-00 1
Stopwatch, digital, 1/100 s 03071-01 1
Water, distilled 5 l 31246-81 1
Universal clamp with joint 37716-00 1
Pipette dish 36589-00 1
D (+)-Sucrose 100 g 30210-10 1
D(+)-Lactose, powder 100 g 31577-10 1
Supp.rod stainl.st.,50cm,M10-thr. 02022-20 1
Volumetric flask 500 ml, IGJ19/26 36551-00 1
Pipettor 36592-00 1
Universal clamp 37715-00 3
Crystallizing dish, boro3.3, 1000ml 46245-00 1
Pasteur pipettes, 250 pcs 36590-00 1
Weighing dishes, square shape, 84 x 84 x 24 mm, 25 pcs. 45019-25 1
Volumetric flask 50 ml, IGJ12/21 36547-00 2
Right angle clamp 37697-00 4
Rubber caps, 10 pcs 39275-03 1
Glass beaker DURAN®, tall, 100 ml 36002-00 10
Volumetric pipette, 10 ml 36578-00 7
Spoon, special steel 33398-00 1
Funnel, glass, top dia. 55 mm 34457-00 1
Magnetic stirring bar 30 mm, cylindrical 46299-02 1
Wash bottle, plastic, 500 ml 33931-00 1
Magn.stirring bar 15mm, cyl. 46299-01 1
Rubber tubing, i.d. 6 mm 39282-00 2
Hose clip, diam. 8-16 mm, 1 pc. 40996-02 3
Laboratory Experiments Physics, Chemistry, Biology andApplied Sciences, CD-ROM, incl. operating manuals 16502-42 1

Literature for this article als follows

Title Product Language
TESS expert Handbook Laboratory Experiments Chemistry 16504-12 ENG

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Product No: P3050301

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