Substrate inhibition of enzymes (with the Cobra3 Chem-Unit)

Principle

The enzymatic hydrolysis of urea in aqueous solution liberates carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution.

Tasks

1. Conductivity measurements enable the rate of hydrolysis of urea by the enzyme urease to be determined at various substrate concentrations. Inhibition of the enzyme by the substrate occures at excessive substrate concentrations.

What you can learn about

• Substrate inhibition
• Enzymolysis of urea
• Conductivity-time plot
• Reaction velocity of enzymatic hydrolysis

Literature for this article als follows

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