Enzyme kinetics: Inhibition and poisoning of enzymes (with the Cobra3 Basic-Unit)

Product No: P4120511

Principle

The enzymatic hydrolysis of urea in aqueous solutions liberates carbon dioxide and ammonia. The ions of these compounds increase the conducitivity of the solution.

Tasks

  1. Conductivity measurements enable the rate of hydrolysis of urea by the enzyme urease to be determined. The addition of an appropriate inhibitor poisons the enzyme, so that it no longer converts substrate.

 What you can learn about

  • Poisoning of enzymes
  • Enzymatic hydrolysis of urea
  • Silver nitrate inhibition of urease
  • Conducivitiy measurement



Materiallist (Excerpt) Product Amount
Cobra3 BASIC-UNIT, USB 12150-50 1
Measuring module Conductivity 12108-00 1
Conductivity probe K1 18151-02 1
Urease soln.in 50% glycerol,10ml 31924-03 1
Magnetic stirrer Mini / MST 47334-93 1
Cobra3, sensor -20..110 C 12120-00 1
Micro-l syringe, 100 micro-l 02606-00 1
Silver nitrate, cryst. 15 g 30222-00 1
Power supply 12V / 2A 12151-99 1
Support base variable 02001-00 1
Software Cobra3 Conductivity 14508-61 1
Water, distilled 5 l 31246-81 1
Urea, 250 g 30086-25 1
Separator f.magnetic bars 35680-03 1
Pipettor 36592-00 1
Universal clamp 37718-00 1
Boss head 02043-00 1
Pasteur pipettes, 250 pcs 36590-00 1
Support rod, stainless steel, 500 mm 02032-00 1
Rubber caps, 10 pcs 39275-03 1
Glass beaker DURAN®, tall, 100 ml 36002-00 8
Volumetric pipette, 20 ml 36579-00 1
Microspoon, steel 33393-00 1
Magnetic stirring bar 30 mm, cylindrical 46299-02 1
Wash bottle, plastic, 500 ml 33931-00 1
Volumetric pipette, 1 ml 36575-00 1
Beaker, 250 ml, low form, plastic 36013-01 1

Literature for this article als follows

Title Product Language
TESS expert Handbook Laboratory Experiments Biology 16506-02 ENG

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Product No: P4120511

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