Product details

Chromatographic separation processes: Gas chromatography with Cobra4

Chromatographic separation processes: Gas chromatography with Cobra4

Item no.: P3031760

Difficulty Expenditure of Time print
  • Description
  • Scope of Supply
  • Accessories
  • Downloads and Documents

Principle

Chromatographic procedures allow a separation of substance mixtures with the aid of a stationary separation phase and a mobile phase. In gas chromatography the mobile phase is a gas. The mobile phase, to which the mixture to be separated is added, transports the substance mixture through the separation column at a constant flow rate. Interactions occur between the mobile phase and the stationary phase. The establishment of equilibria between the stationary phase and the different substances (distribution equilibria, adsorption-desorption equilibria) results in different migration rates of the individual components. At the end of the column there is a detector in the form of a thermal conductivity cell, which can detect the different substances on the basis of their differing thermal conductivities. The detector signal is recorded as a funtion of time. The different thermal conductivities of the carrier gas and the substance cause temperature alterations in the electrically heated temperature sensor, which is located in a Wheatstone bridge circuit. The resulting electrical signal is recorded by a plotter as a function of time (chromatogram).

Benefits

  • Didactically demonstrative setup
  • Simplified implementation: all pre-settings already prepared
  • Glass jacket system easily expandable
  • Many different gases separable

Tasks

  1. Determine the retention times of different gases and perform a chromatographic material separation of a mixture of butane gases.
  2. Separate and identify the components of a two-component mixture consisting of ethanol and ethyl acetate chromatographically.

What you can learn about

  • Chromatography
  • Chromatogram
  • Multiplicative distribution
  • Nernst's law of distribution (number of theoretical trays)
  • Thermal conductivity detector

Necessary accessories

  • Precision balance 620g/0.001g

Software provided. Computer not included.

Device Name Item no. Qty
USB charger for Cobra4 Mobile-Link 2 and Wireless/USB-Link 07932-99 1,00
Bath for thermostat, makrolon 08487-02 1,00
External circulation set for thermostat Alpha A 08493-02 1,00
Immersion thermostat Alpha A, 230 V 08493-93 1,00
Cobra4 Wireless/USB-Link incl. USB cable 12601-10 1,00
Cobra4 Sensor-Unit Chemistry 12630-00 1,00
Holder for sensors with support rod 12680-00 1,00
Acetone, chemical pure, 250 ml 30004-25 1,00
Ethyl alcohol, absolute 500 30008-50 1,00
Ethyl acetate 250 30075-25 1,00
Water, distilled 5 l 31246-81 1,00
Quartz glass wool 10 g 31773-03 1,00
Butane burner, Labogaz 206 type 32178-00 1,00
Spoon, special steel 33398-00 1,00
Reducing valve for CO2 / He 33481-00 1,00
Fine control valve 33499-00 1,00
Snap-cap vials,d=24mm,h=52mm,10p. 33621-03 2,00
Dish, plastic, 150x150x65 mm 33928-00 1,00
Security bottle with manometer and three-way stopcock 34170-88 1,00
Funnel, glass, top dia. 50 mm 34457-00 1,00
Connecting tube,IGJ29/32-GL18/8 35678-02 1,00
Round-bott.flask, 500ml,IGJ 29/32 35862-00 1,00
Beaker, tall form, 150 ml 36003-00 2,00
Pasteur pipettes, 250 pcs 36590-00 1,00
Gas separation column 36670-00 1,00
Measure probe for gas chromatog 36670-10 1,00
Control unit gas chromatograph 36670-99 1,00
Soap bubble flow meter 36675-00 1,00
Glass tube, straight, l=80 mm, 10/pkg. 36701-65 1,00
Right angle boss-head clamp 37697-00 5,00
Universal clamp 37715-00 4,00
Lab thermometer,-10..+150C 38058-00 1,00
Laboratory pen, waterproof, black 38711-00 1,00
Rubber stopper,d=18/14mm,w/o hole 39254-00 1,00
Rubber caps, 10 pcs 39275-03 1,00
Rubber tubing, i.d. 6 mm 39282-00 6,00
Rubber tubing,vacuum,i.d.6mm 39286-00 2,00
Lab protecting glasses with UV filter 39315-00 1,00
Wrench for steel cylinders 40322-00 1,00
Gas bar 40466-00 2,00
Hose clip, diam. 8-16 mm, 1 pc. 40996-02 7,00
Hose clamp for 15-22 mm diameter 40999-00 2,00
Stopper,IGJ 29/32,glass,clear 41256-10 1,00
Compressed gas, iso-butane, 490 g 41773-12 1,00
Table stand for 2 l steel cylinders 41774-00 1,00
Steel cylinder helium, 2 l, filled 41776-00 1,00
Teflon sleeve IGJ 29, 10 pcs 43617-00 1,00
Gloves, Neoprene, medium 46347-00 1,00
Tubing connector, ID 6-10mm 47516-01 2,00
Support base DEMO 02007-55 1,00
Support rod, stainless steel, 750 mm 02033-00 2,00
Support clamp for small case 02043-10 1,00
Syringe 1ml, Luer, 10 pcs 02593-03 1,00
Cannula 0,45x13 mm, Luer, 20 pcs 02598-04 1,00
Microliter syringe, 10 micro-l 02607-00 1,00
Glass jacket 02615-00 1,00
Water jet pump, plastic 02728-00 1,00
Stop clock, demo.; diam. 13 cm 03075-00 1,00
Connecting cord, 32 A, 750 mm, red 07362-01 1,00
Connecting cord, 32 A, 750 mm, blue 07362-04 1,00
Adapter, BNC-plug/socket 4 mm 07542-26 1,00
Butane cartridge C206, without valve, 190 g 47535-01 1,00
curricuLAB measureLAB 14580-61 1,00

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