Product details

Column chromatography - separation of leaf pigments

Column chromatography - separation of leaf pigments

Item no.: P3120300

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Principle

In this investigation, a uniformly green raw extract of fresh leaves is first separated into different fractions by means of column chromatography. To do so, the extract is added to a column filled with starch and drawn through the column under slightly reduced pressure (to increase the flow rate of the mobile phase) with ligroin as the eluent. A separation occurs in a clearly recognisable, broad, yellow area and in a narrow, green band. This means that the xanthophylls (yellow) are separated from the chlorophylls (green). If the vacuum is reduced during the separation, the separation is much better, but then separation also takes considerably longer. Each of the separation fractions can be collected individually and characterised by recording their absorption spectra, if necessary, or examined for fluorescence by radiation with UV light.

Benefits

  • Optimised for demonstration experiments: Transformation from horizontal into vertical direction
  • Practical water jet pump for easy generation of the required negative pressure
  • Didactic introduction to a basic separation process

Tasks

Investigate different leaf pigments using column chromatography.

What you can learn about

  • Chlorophyll
  • Column chromatography
  • Leaf pigments
  • Xanthophyll
Device Name Item no. Qty
Glass tubes, right-angled, 10 36701-57 1,00
Spring manometer, 0...-1000 mbar 34170-02 1,00
Sea sand, purified 1000 30220-67 1,00
Pipette, w. rubber bulb, long tip 64838-00 1,00
Frame for complete experiments 45500-00 1,00
Erlenmeyer flask, GL 25/12, 100ml 35844-15 4,00
Mortar w. pestle, 325 ml, porcelain 32605-00 1,00
Petroleum ether, 40-60 °C, 500 ml 30184-50 1,00
Clamping holder,turnable,8-10mm 45522-00 1,00
Rubber tubing,vacuum,i.d.6mm 39286-00 3,00
Graduated pipette, 0.1 ml 36594-00 1,00
Funnel, glass, top dia. 80 mm 34459-00 1,00
Starch,soluble 100 30227-10 1,00
G-clamp 02014-00 2,00
Rear-cover for compl.-exp. panel 45501-00 1,00
Stopcock,3-way,t-shaped, glass 36731-00 1,00
Erlenm.flask wide neck 250ml PN45 36434-00 1,00
Circular filter,d 110 mm,100 pcs 32977-04 1,00
Spring plugs, 50 off 45530-00 1,00
Glass rod,boro 3.3,l=300mm, d=9mm 40485-07 1,00
Graduated cylinder, borosilicate, 50 ml 36628-00 1,00
Column for ion-exchange chromatography 35025-01 1,00
Clamp on holder 02164-00 1,00
Panel for complete experimental setups 45510-00 1,00
Air control valve 37003-00 1,00
Volumetric pipette, 50 ml 36581-00 1,00
Secure bottle, 500 ml, 2 x Gl 18/8, 1 x 25/12 34170-01 1,00
Scissors, straight,180 mm 64798-00 1,00
Closure caps,10, GL25 41221-03 1,00
Glass tube, right-angled 36701-07 1,00
Glass wool 10 g 31773-03 1,00
Water jet pump, plastic 02728-00 1,00
Clamping holder,18-25mm 45520-00 2,00
Universal clamp 37715-00 1,00
Pipettor 36592-00 1,00
Propyl alcohol,normal 250 31754-25 1,00
Document     Filesize
p3120300e.pdf Experiment guide, English 258.26 KB

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