Product details

Determination of the Michaelis constant with Cobra4

Determination of the Michaelis constant with Cobra4

Item no.: P4120360

Experiment Type: Teacher Experiment, Laboratory Experiment

Difficulty Expenditure of Time print
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  • Scope of Supply
  • Downloads and Documents

Principle

The enzymatic hydrolysis of urea in aqueous solution liberates carbon dixide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements can so be made to determine the rate of hydrolysis of urea by the enzyme urease at various substrate concentrations.

Benefits

  • Compact, easily transportable setup
  • For both demonstration and student experiments
  • Flexible experimenting thanks to wireless data transmission
  • Additional experiment in plant physiology and biochemistry can be performed with few modifications
  • Data acquisition with tablets possible
  • With detailed experiment guide

Tasks

  • Measure conductivity changes of urea solutions of different concentrations brought about by the activity of urease and calculate the Michaelis constant

What you can learn about

  • Michaelis constant
  • Enzymatic hydrolysis of urea
  • Conductivity measurement
  • Bodenstein principle
  • Enzyme-substrate complex
  • Lineweaver-Burk plot

Software included. Computer not provided.

Document     Filesize
p4120360e.pdf Experiment guide, English 1.58 MB

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