Product details

Enzyme inhibition (poisoning of enzymes) with Cobra4

Enzyme inhibition (poisoning of enzymes) with Cobra4

Item no.: P4120560

Experiment Type: Teacher Experiment, Laboratory Experiment

Difficulty Expenditure of Time print
  • Description
  • Scope of Supply
  • Downloads and Documents

Principle

The enzymatic hydrolysis of urea in aqueous solutions liberates carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution.

Benefits

  • Compact, easily transportable setup
  • For both demonstration and student experiments
  • Flexible experimenting thanks to wireless data transmission
  • Additional experiment in plant physiology and biochemistry can be performed with few modifications
  • Data acquisition with tablets possible
  • With detailed experiment guide

Tasks

  1. Conductivity measurements enable the rate of hydrolysis of urea by the enzyme urease to be determined.
  2. The addition of an appropriate inhibitor poisons the enzyme, so that it no longer converts substrate.

What you can learn about

  • Poisoning of enzymes
  • Enzymatic hydrolysis of urea
  • Silver nitrate inhibition of urease
  • Conducivitiy measurement

Software included. Computer not provided.

Document     Filesize
p4120560e.pdf Experiment guide, English 1.03 MB

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