Principle
The enzymatic hydrolysis of urea in aqueous solutions liberates
carbon dioxide and ammonia. The ions of these compounds increase
the conductivity of the solution.
Benefits
- Compact, easily transportable setup
- For both demonstration and student experiments
- Flexible experimenting thanks to wireless data transmission
- Additional experiment in plant physiology and biochemistry can be performed with few modifications
- Data acquisition with tablets possible
- With detailed experiment guide
Tasks
- Conductivity measurements enable the rate of hydrolysis of urea
by the enzyme urease to be determined.
- The addition of an appropriate inhibitor poisons the enzyme, so
that it no longer converts substrate.
What you can learn about
- Poisoning of enzymes
- Enzymatic hydrolysis of urea
- Silver nitrate inhibition of urease
- Conducivitiy measurement
Software included. Computer not provided.