Principle
The enzymatic hydrolysis of urea in aqueous solution liberates carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Inhibition of the enzyme by the substrate occures at excessive substrate concentrations.
Benefits
- Compact, easily transportable setup
- For both demonstration and student experiments
- Flexible experimenting thanks to wireless data transmission
- Additional experiment in plant physiology and biochemistry can be performed with few modifications
- Data acquisition with tablets possible
- With detailed experiment guide
Tasks
- Measurement of conductivity to determine the rate of hydrolysis of urea by the enzyme urease at various substrate concentrations
What you can learn about
- Substrate inhibition
- Enzymolysis of urea
- Conductivity-time plot
- Reaction velocity of enzymatic hydrolysis
Software included. Computer not provided.
1 year warranty