The enzymatic hydrolysis of urea in aqueous solution yields carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements can be used to determine the rates of urea hydrolysis by the enzyme urease at different substrate concentrations. Excessive substrate concentrations lead to substrate inhibition.
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Measurement of conductivity and determination of the rate of urea hydrolysis by the enzyme urease at different substrate concentrations
- substrate inhibition
- urea enzymolysis
- conductivity diagram
- enzyme hydrolysis reaction rate