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Technical data


Protein containing nutrients

Article no: P1256101

Principle

In this experiment several basic operations of the Kjeldahl method are experimental simulated. Proteins are detected by using copper sulfate and sodium hydroxide solution.

Proteins differs from carbohydrates and fats by containing nitrogen.  Protein can be detected by cooking the sample or using the Buiret-test, whereby the Biuret-test is better suited for the detection of protein than cooking the sample.

After the addition of copper sulfate and sodium hydroxide solution into an egg-solution, a color change can be observed to violet. The color change occur, because the copper ions form a colored complex compound with the nitrogen atoms of the proteins , which can be recognized by its characteristic violet color.

 

Learning objectives

  • Buiret-test
  • Detecion of proteins

 

Tasks

Learn to recognize possible tests for protein and examine various foods for protein.

 

Scope of delivery

Mortar w. pestle, 70ml, porcelain 32603-00 1
Round filters, 100 pcs., various sizes 32977-03 1
Knife, stainless 33476-00 1
Funnels, plastic, various sizes 47318-00 1
Watch glass, dia.60 mm 34570-00 3
Beakers, Boro, high form, various sizes 46026-00 1
Beaker, 250 ml, plastic (PP) 36082-00 1
Graduated cylinders, plastic, various sizes 36629-01 1
Test tube, laboratory glass, various lengths, 100 pcs. 37656-10 5
Test tube holder, up to d 22mm 38823-00 1
Spoon, with spatula end, 180 mm, plastic 38833-00 1
Protecting glasses, clear glass 39316-00 1
Glass rod, boro 3.3, l=200mm, d=5mm 40485-03 1
Wide-mouth flasks, clear, narrow neck, with stopper, various sizes 41101-01 4
Dissecting needle, pointed 64620-00 1
Pipette with rubber bulb 64701-00 2
Water, distilled 5 l 31246-81 1
Sodium chloride, 500 g 30155-50 1
Peptone,dry,from meat 50 g 31708-05 1
Sodium hydroxide, pellets, 500 g 30157-50 1
Copper-II sulphate,cryst. 250 g 30126-25 1
Butane burner, Labogaz 206 type 32178-00 1
Butane cartridge C206, without valve, 190 g 47535-01 1
Portable Balance, OHAUS CR221, 220 g / 0.1 g 48913-00 1

PHYWE Systeme GmbH & Co. KG
Robert-Bosch-Breite 10 – 37079 Göttingen – Germany
www.phywe.com