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Technical data


Determination of the Michaelis constant with Cobra SMARTsense

Article no: P4120369

Principle

The enzymatic hydrolysis of urea in aqueous solution yields carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements can be used to determine the rates of urea hydrolysis by the enzyme urease at different substrate concentrations. The Michaelis constant can be calculated from these values.

Benefits

  • Especially understandable and didactically prepared description of the experiment (reference to everyday life etc.) including protocol questions
  • Future-oriented teaching: Integration into digital science lessons with tablets or smartphones
  • Increased motivation of students by using the intuitive measureAPP software
  • Compact, easily transportable experimental set-up
  • Suitable for demo experiments and for lab course experiments
  • Flexible test execution thanks to wireless data transmission

Tasks

  1. Measurement of the change in conductivity due to urease activity in solutions with different urea concentrations and calculation of the Michael constant

Learning objectives

  • Michaelis constant
  • Enzymatic urea hydrolysis
  • Conductivity measurement
  • Soilstone principle
  • Enzyme-substrate complex
  • Lineweaver-Burk-Plot

 

 

 

Scope of delivery

Cobra SMARTsense - Conductivity, 0...20000 µS/cm, 0...60°C (Bluetooth) 12922-01 1
Separator for magnetic bars 35680-03 1
Magnetic stirrer with heating, stainless steel, digital, 280 °C, 100-1500 rpm FHO-RSM10HS 1
Magnetic stirring bar, 50 mm, cylindrical 46299-03 1
Portable Balance, OHAUS YA302 49213-00 1
Retort stand, h = 750 mm 37694-00 1
Boss head 02043-00 1
Universal clamp 37715-00 1
Beakers, Boro, high form, various sizes 46026-00 1
Beaker, 250 ml, plastic (PP) 36013-01 1
Erlenmeyer flasks, Boro, narrow neck, various sizes 46141-00 1
Rubber stoppers, without bore, various diameters 39255-00 6
Bulb pipettes, various sizes 36579-00 1
Bulb pipettes, various sizes 36581-00 1
Pipettor,bulb,3 valves,100ml max. 47127-02 1
Micro-l syringe, 100 micro-l 02606-00 1
Spoon, special steel 33398-00 1
Wash bottle, plastic, 500 ml 33931-00 1
Urea, 250 g 30086-25 1
Urease, 5 g 31924-02 1
Water, distilled 5 l 31246-81 1
measureAPP - the free measurement software for all devices and operating systems 14581-61 1

PHYWE Systeme GmbH & Co. KG
Robert-Bosch-Breite 10 – 37079 Göttingen – Germany
www.phywe.com