Forensic PCR experiment kit

Function and Applications

This introductory PCR lab will help students gain confidence with important lab techniques such as micropipetting, PCR, and gel electrophoresis. In the process, they will explore the hereditary disease cystic fibrosis and the connections between genes and disease, personal identification, and the use of biotechnology for genetic analysis.

Equipment and Technical Data

Scope of delivery:

• 2X EZ PCR Master Mix, Load-Ready™

• 3X Crime Lab Primer Mix

• 4 different DNA samples to investigate, including controls

• 100 bp DNA ladder, Load-Ready™

Storage:

• If kept in the freezer, reagents can be stored for 12 months after receipt.
• If kept in the refrigerator, reagents can be stored for 1 month after receipt.

Note:

• Time required for PCR preparation is 30 minutes.
• Time required for electrophoresis is 30 minutes.
• PCR can be performed unattended and takes a max. of 2 hours, whereupon the PCR products can be left at room temperature for up to one week in the thermocycler.

This kit does not include the reagents for the electrophoresis step.

Required accessories:

• Microliter pipette 2-20 µl (47141-10)
• Microliter pipette 20-200 µl (47141-11)
• Both 1.5 ml and 0.2 ml tubes (37653-00 and 35928-01)
• Thermocycler miniPCR for 8 resp. 16 samples (35013-99 resp. 35014-99)
• For subsequent electrophoresis to visualize the results you need a gel electrophoresis system, e.g. blueGel (35017-99) and SeeGreen™ Agarose Tabs (35018-71).