(function () { function textOf(el) { return (el && el.textContent || "").replace(/\s+/g, " ").trim(); } function resolveItemUrl() { var match = window.location.search.match(/[?&]item=(\d+)/); if (!match) { return null; } return window.location.origin + "/a-" + match[1] + "/"; } function loadAccordion() { var url = resolveItemUrl(); if (!url) { return Promise.resolve(null); } return new Promise(function (resolve, reject) { var xhr = new XMLHttpRequest(); xhr.open("GET", url, true); xhr.onload = function () { if (xhr.status < 200 || xhr.status >= 400) { reject(new Error("item page " + xhr.status)); return; } var doc = new DOMParser().parseFromString(xhr.responseText, "text/html"); resolve(doc.querySelector(".phywe-item-accordion")); }; xhr.onerror = function () { reject(new Error("item page network error")); }; xhr.send(); }); } function parseItemBlocks(scope, withQuantity) { if (!scope) { return []; } var rows = scope.querySelectorAll(".itemslist .row.itemblocks"); var items = []; for (var i = 0; i < rows.length; i++) { var cols = rows[i].children; if (!cols.length) { continue; } var nameLink = cols[0].querySelector("a"); var numberLink = cols[1] ? cols[1].querySelector("a") : null; var item = { name: textOf(nameLink || cols[0]), mappedTo: textOf(numberLink) }; if (withQuantity && cols[2]) { var qty = parseInt(textOf(cols[2]).replace(/[^\d]/g, ""), 10); item.quantity = isNaN(qty) ? textOf(cols[2]) : String(qty); } if (item.name) { items.push(item); } } return items; } function fillTable(tableId, items, withQuantity) { var table = document.getElementById(tableId); if (!table || !items.length) { return; } var tbody = table.querySelector("tbody"); for (var i = 0; i < items.length; i++) { var tr = document.createElement("tr"); var nameTd = document.createElement("td"); nameTd.style.width = "80%"; nameTd.textContent = items[i].name; tr.appendChild(nameTd); var numberTd = document.createElement("td"); numberTd.textContent = items[i].mappedTo; tr.appendChild(numberTd); if (withQuantity) { var qtyTd = document.createElement("td"); qtyTd.textContent = items[i].quantity || ""; tr.appendChild(qtyTd); } tbody.appendChild(tr); } var wrapper = table.parentElement; while (wrapper && wrapper.className.indexOf("printtablediv") === -1) { wrapper = wrapper.parentElement; } if (wrapper) { wrapper.style.display = ""; } } function stepTitle(stepEl) { if (!stepEl) { return ""; } var titleEl = stepEl.querySelector(".card-header button div") || stepEl.querySelector(".card-header button"); return textOf(titleEl); } function setHeadline(tableId, title) { var headline = document.querySelector("#" + tableId + " .printtableheadline"); if (headline && title) { headline.textContent = title; } } function parseMedia(scope) { if (!scope) { return []; } var rows = scope.querySelectorAll(".itemslist .row.itemblocks"); var items = []; for (var i = 0; i < rows.length; i++) { var cols = rows[i].children; if (!cols.length) { continue; } var nameLink = cols[0].querySelector("a"); var fileLink = cols[1] ? cols[1].querySelector("a") : null; var item = { name: textOf(nameLink || cols[0]), mappedTo: textOf(fileLink || cols[1]), quantity: cols[2] ? textOf(cols[2]) : "" }; if (item.name) { items.push(item); } } return items; } function fillFromAccordion(accordion) { if (!accordion) { return; } var versucheStep = accordion.querySelector('[data-step="versuche"]'); var versuche = parseItemBlocks(versucheStep, false); setHeadline("print-versuche", stepTitle(versucheStep)); fillTable("print-versuche", versuche, false); var lieferumfangStep = accordion.querySelector('[data-step="lieferumfang"]'); var lieferumfang = parseItemBlocks(lieferumfangStep, true); fillTable("print-lieferumfang", lieferumfang, true); var zubehoer = accordion.querySelector('[data-step="zubehoer"]'); var sections = zubehoer ? zubehoer.querySelectorAll(".card-body") : []; var notwendig = []; var empfohlen = []; for (var i = 0; i < sections.length; i++) { var items = parseItemBlocks(sections[i], false); if (sections[i].querySelector(".text-red, .icon-warning-black")) { notwendig = items; } else if (sections[i].querySelector(".text-green, .icon-Mehrwert_black")) { empfohlen = items; } } fillTable("print-zubehoer-notwendig", notwendig, false); fillTable("print-zubehoer-empfohlen", empfohlen, false); var mediaStep = accordion.querySelector('[data-step="media"]'); var media = parseMedia(mediaStep); setHeadline("print-media", stepTitle(mediaStep)); fillTable("print-media", media, true); } function start() { var itemUrl = resolveItemUrl(); if (!itemUrl) { setTimeout(function () { window.print(); }, 2000); return; } loadAccordion() .then(function (accordion) { fillFromAccordion(accordion); }) .catch(function (err) { if (window.console && console.warn) { console.warn("Accordion konnte nicht geladen werden:", resolveItemUrl(), err); } }) .then(function () { setTimeout(function () { window.print(); }, 500); }); } if (document.readyState === "loading") { document.addEventListener("DOMContentLoaded", start); } else { start(); } })();
 

Technical data


Polymerase chain reaction (PCR)

Article no: P8111100
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> Unknown

Principle

This experiment is designed to teach PCR laboratory techniques in a forensic context. In addition, students also learn about the hereditary disease cystic fibrosis and the links between genes and disease, personal identification and the use of biotechnology for genetic analysis.

 

Benefits

  • Complete set including all necessary consumables

  • Consumables can be purchased separately as a kit

  • Ready to use: the DNA samples can be used directly in the PCR with minimal effort

  • Well-documented, simple experiment procedure

 

Tasks

  1. Sample preparation for PCR

  2. Creating a PCR programme on a thermocycler

  3. Performing a PCR

  4. Sample preparation for analysing the band patterns after gel electrophoresis

 

Learning objectives

  • PCR and its laboratory techniques, such as micropipetting

  • PCR as tool of acquiring evidence in forensics

  • The hereditary disease cystic fibrosis

 

To perform subsequent gel electrophoresis, a gel electrophoresis device is required. We recommend the student set TESS Genetics (15312-88).

 

 


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