Technical data

Polymerase chain reaction (PCR) with own DNA

Article no: P8111200

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Principle

Students use their own DNA to participate in an authentic investigation on the genetics of sleep and circadian clocks. Use PCR to determine your own genotype at a locus that has been associated with morning vs. evening preference.

Benefits

Complete set including all necessary consumables
Consumables can be purchased separately as a kit
Ready to use: the DNA samples can be used directly in the PCR with minimal effort
Well-documented, simple experiment procedure

Tasks

Sample preparation for PCR
Creating a PCR programme on a thermocycler
Performing a PCR
Sample preparation for analysing the band patterns after gel electrophoresis

Learning objectives

Sample preparation of own DNA for use in PCR
PCR and its laboratory techniques, such as micropipetting
Determination of individual sleep preference
Genetics of sleep

To perform subsequent gel electrophoresis, a gel electrophoresis device is required. We recommend the student set TESS Genetics (15312-88).

Scope of delivery

miniPCR mini16X Thermocycler for 16 samples, PCR machine	35014-99	1
Sleep Lab: Lark or Owl: PCR experiment kit using your own DNA	35018-04	1
PCR single tubes, 0.2 ml, pack of 100, in bag	35928-01	1
Microtubes, disposable, 1.5ml, 50/pkg	37653-02	1
Rack for 8 x 0.2 ml disposable reaction tubes for PCR	37652-01	1
Rack for disposable 1.5 ml reaction tubes	37652-00	1
Microliterpipette 2-20 µl, autoclavable	47141-10	1
Microliterpipette 20-200 µl, autoclavable	47141-11	1
Pipette tips, 2-200 µl, 96 tips, racked	47148-11	1

Necessary accessories

Student set Genetics, TESS advanced Biology

15312-88