Column chromatography - separation of leaf pigments  ![]()
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Principle In this investigation, a uniformly green raw extract of
fresh leaves is first separated into different fractions
by means of column chromatography. To do so, the
extract is added to a column filled with starch and drawn
through the column under slightly reduced pressure (to increase the
flow rate of the mobile phase) with ligroin as the eluent. A
separation occurs in a clearly recognisable, broad, yellow area and
in a narrow, green band. This means that the xanthophylls (yellow)
are separated from the chlorophylls (green). If the vacuum is
reduced during the separation, the separation is much better, but
then separation also takes considerably longer. Each of the
separation fractions can be collected individually and
characterised by recording their absorption spectra, if necessary,
or examined for fluorescence by radiation with UV light. Benefits - Optimised for demonstration experiments: Transformation from horizontal into vertical direction
- Practical water jet pump for easy generation of the required negative pressure
- Didactic introduction to a basic separation process
Tasks Investigate different leaf pigments using column
chromatography. Learning objectives - Chlorophyll
- Column chromatography
- Leaf pigments
- Xanthophyll
Scope of delivery |
Frame for complete experiments
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45500-00
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1
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Rear-cover for compl.-exp. panel
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45501-00
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1
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Panel for complete experimental setups
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45510-00
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1
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Clamping holder,18-25mm
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45520-00
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2
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Clamping holder,turnable,8-10mm
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45522-00
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1
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Clamp on holder
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02164-00
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1
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Universal clamp
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37715-01
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1
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Spring plugs, 50 off
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45530-00
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1
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G-clamp
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02014-01
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2
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Column for ion-exchange chromatography
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35025-01
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1
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Vacuum adaptor, straight, GL25/12
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MAU-27228500
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1
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Erlenmeyer flask, GL 25/12, 100ml
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MAU-27228600
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4
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Secure bottle, 500 ml, 2 x Gl 18/8, 1 x 25/12
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34170-01
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1
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Spring manometer, 0...-1000 mbar
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34170-02
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1
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Glass tubes, right-angled, 150 x 80 mm
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MAU-10022500
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1
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Stopcock,3-way,t-shaped, glass
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36731-00
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1
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Air control valve
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37003-00
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1
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Mortar w. pestle, 325 ml, porcelain
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32605-00
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1
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Funnels (glass), Boro, various sizes
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34459-00
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1
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Round filters, 100 pcs., various sizes
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32977-04
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1
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Erlenmeyer flasks with ground joint, Duran®, various volumes
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36434-00
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1
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Pipettes with rubber cap, various sizes
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64838-00
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1
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Graduated cylinders, Boro, various sizes
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36628-00
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1
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Bulb pipettes, various sizes
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36581-00
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1
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Graduated pipette, 5 ml : 0,05
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36594-00
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1
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Pipettor
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36592-00
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1
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Glass rod,boro 3.3,l=300mm, d=9mm
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40485-07
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1
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Water jet pump, plastic
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02728-00
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1
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Scissors, straight, 180 mm
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64798-00
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1
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Rubber hose for vacuum, various diameters
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39286-00
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3
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Glass wool 10 g
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31773-03
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1
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Sea sand, purified 1000 g
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30220-67
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1
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Starch,soluble 100 g
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30227-10
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1
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Petroleum ether, 40-60 °C, 500 ml
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30184-50
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1
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Propyl alcohol,normal 250 ml
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31754-25
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1
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Service
