Electrophoresis of Lambda DNA

Article no. P8110200 | Type: Experiments

20 Minutes
45 Minutes
grades 10-13 , University
Pupils
heavy

Principle

The most important work methods of molecular genetics are taught in this experiment. With the aid of agarose gel electrophoresis migration velocity and the cleavage pattern of lambda DNA can be investigated.

Benefits

  • Complete equipment set which also contains all required consumables
  • Consumables can be purchased as a replacement kit
  • Ready to use: DNA fragment samples are already predigested
  • Easy to use: detailed experiment guide
  • DNA samples can can be stored for a longer period at room temperature because they are lyophilized

Tasks

  • Cast the agarose gel
  • Prepare samples for electrophoresis
  • Perform electrophoresis
  • Stain the samples and analyse the banding pattern

Learning objectives

  • Lambda DNA
  • Electrophoresis
  • Restriction enzymes
  • Restriction digestion
  • Banding pattern

Name
File name
File size
File type
(de) Versuchsbeschreibung
p8110200d .pdf
File size 0.35 Mb
pdf
(en) Experiment guide
p8110200e .pdf
File size 0.40 Mb
pdf
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