Polymerase chain reaction (PCR) with own DNA

Principle

Students use their own DNA to participate in an authentic investigation on the genetics of sleep and circadian clocks. Use PCR to determine your own genotype at a locus that has been associated with morning vs. evening preference.

Benefits

• Complete set including all necessary consumables

• Consumables can be purchased separately as a kit

• Ready to use: the DNA samples can be used directly in the PCR with minimal effort

• Well-documented, simple experiment procedure

Tasks

1. Sample preparation for PCR

2. Creating a PCR programme on a thermocycler

3. Performing a PCR

4. Sample preparation for analysing the band patterns after gel electrophoresis

Learning objectives

• Sample preparation of own DNA for use in PCR

• PCR and its laboratory techniques, such as micropipetting

• Determination of individual sleep preference

• Genetics of sleep

To perform subsequent gel electrophoresis, a gel electrophoresis device is required. We recommend the student set TESS Genetics (15312-88).