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Function and Applications
Student experiment kit for conducting 8 experiments in digestive enzymology:
- Fat digestion
- Effect of bile
- Fat digestion with the participation of bile
- Digestion of carbohydrates - Detection of starch and glucose
- Starch degradation by amylase
- Detection of starch degradation products
- Protein digestion by pepsin
- Protein digestion by pancreatin
Benefits
- Complete kit containing the necessary enzymes, chemical substances, and materials for conducting multiple experiments.
- Minimal preparation time and easy experiment setup with detailed instructions.
Technical Specification
- alpha-amylase, powder, 1 g
- Pepsin, powder, 5 g
- Pancreatin, 10 g
- Olive oil, 100 ml
- Phenolphthalein solution, 0.1%, 50 ml
- Ox bile, 25 g
- Starch, 25 g
- D(+)-glucose, 250 g
- Iodine-potassium iodide solution (Lugol’s solution), 100 ml
- Gelatin, powder, 50 g
- Casein, alkali-soluble, 100 g
- Cotton wool, white, 200 g
- Microscale spatula, steel, l = 150 mm
- 2 x measuring pipette, 10 ml, graduation 0.1 ml
- 4 x measuring pipette, 5 ml, graduation 0.02 ml
- Pipetting aid, up to 10 ml
The enzymes must be stored at 4°C.
Hazard Class and Hazard Category
- α-Amylase, Resp. Sens. 1 H334 – May cause allergy, asthma-like symptoms, or breathing difficulties if inhaled.
- Lugol’s solution, STOT RE 2 H373 – May cause damage to organs through prolonged or repeated exposure.
- Pancreatin, Skin Irrit. 2 H315 – Causes skin irritation. Eye Irrit. 2 H319 – Causes serious eye irritation. STOT SE 3 H335 – May cause respiratory irritation. Resp. Sens. 1 H334 – May cause allergy, asthma-like symptoms, or breathing difficulties if inhaled. Skin Sens. 1 H317 – May cause an allergic skin reaction.
- Pepsin, Eye Irrit. 2 H319 – Causes serious eye irritation. STOT SE 3 H335 – May cause respiratory irritation. Skin Irrit. 2 H315 – Causes skin irritation. Resp. Sens. 1 H334 – May cause allergy, asthma-like symptoms, or breathing difficulties if inhaled.
- Phenolphthalein 0.1% in ethanol, Flam. Liq. 2 H225 – Liquid and vapor are highly flammable. Eye Irrit. 2 H319 – Causes serious eye irritation.
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